The influence of selenium deficiency on chosen biochemical parameters and histopathological changes in muscles of goat kids

The research was conducted on 40 young alpine goats (kids) divided into two groups. First group consisted of 20 kids demonstrating clinical signs muscular dystrophy. Second a control and animals that received intramuscular injection (2ml per animal) vitamin E selenium preparation containing in 1ml 50 mg tocopherol acetate, 0.5mg sodium selenite solvent 2nd day life. were clinically examined blood for laboratory analyses sampled three times from 5 their life 10 intervals. In addition, six 24 days old dystrophy subjected to biceps femoris biopsy. Serum total protein, glucose, triglycerides, cholesterol as well AST, CK LDH determined all the animals. activity glutathione peroxidase (GSH-Px) whole serum concentrations deter-mined 6 each group. Total lactate dehydrogenase its separation isoenzymatic fractions collected biopsy material. muscle samples additionally histopathological examination consisting HE staining HBFP detect necrotic fibers. Symptoms began appear first between 17 23 age included tremors limbs, poor posture, stilt gait increased time laying. did not show any symptoms disease during experiment. Hypo-proteinemia, hypoglycemia, reduction elevated triglycerides level associated with lipolysis adipose tissue have been found sick kids. A significant decrease selenium, levels observed significantly higher well. Five isoenzymes obtained electrophoretic tissue. LDH4and LDH5 dominating, increase fraction also observed. Histopathological revealed changes characteristic presence Zenker necrosis.